Associated production of amylases and cellulases through submerged fermentation
DOI:
https://doi.org/10.14295/vetor.v34i1.15874Keywords:
Enzyme production, bioprocesses, bacteria, Hydrolytic enzymesAbstract
The study aims to optimize the homemade production of amylolytic and cellulolytic enzymes by submerged fermentation. Enzyme production was carried out using sugarcane bagasse and triticale flour as substrates for the fermentation medium, using bacteria isolated from agro-industrial residues. Enzymatic extracts were evaluated for amylolytic and cellulolytic activity. The significant variables (p<0.05) in the amylolytic production were the bacteria and the nitrogen source, for cellulolytic production the bacteria, the nitrogen concentration, and the source. Furthermore, the maximum enzymatic activities were obtained at 120h for both studied enzymes. In this sense, fermentation could be ended in 120 h due to the high production of the enzymes of interest (6.02 U/mL for amylolytic activity and 5.52 U/mL for cellulolytic activity). A second submerged fermentation was carried out, fixing the variables that did not show significance according to the Plackett-Burman design and evaluating two concentrations of ammonium sulfate (0.25 and 0.50%). Enzyme production by bacteria A and B showed no statistical difference (p>0.05) between the experiments, demonstrating that using 0.25% ammonium sulfate is preferable. This allowed for determining the optimized conditions for producing and combining bacterial amylases and cellulases at home, using Bacteria B in a culture medium supplemented with 0.25% ammonium sulfate as the nitrogen source.
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